What Does It Mean When Odds Are Negative? Negative numbers (in American money line odds) are reserved for the favorite on the betting line and indicate how much you need to stake to winR$100 you generally need to put down more to winR$100 on the favorite.
The negative number shows how much has to be risked to win aR$100 payout. Thus, if the money line for the team that you picked was -400, it means that if you placed a successfulR$400 bet, you would gainR$100 on payout.
The linear range can be measured simply by making a plot of analyte concentration versus fluorescence, using evenly-spaced analyte concentrations, and seeing at what concentration the data deviate from a straight line that is tangent to the low end of the concentration range.
Determining the linear range is relatively easy, and can be achieved by taking a sample and performing a serial dilution. If the ranges overlap then determining the amount of sample to load is also similarly easy.
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